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The effect of “’A’ Cell Incubator” that places an A-Counter onto itself whenever an A-Counter is removed by a card effect does not use the Chain.
The effect of “’A’ Cell Incubator” that distributes the A-Counters on “’A’ Cell Incubator” and places them onto monsters when it is destroyed begins a Chain.
If an A-Counter on “’A’ Cell Incubator” is removed by an effect, an A-Counter is still placed upon “’A’ Cell Incubator”.
The effect of “’A’ Cell Incubator” is not applied when A-Counters are removed because the card with the A-Counters was removed from the field or flipped face-down, because these A-Counters are not removed by an effect.
If an A-Counter is removed to pay a cost, an A-Counter is placed upon “’A’ Cell Incubator”.